ESTABLISHMENT OF STABLE MULTILINEAGE HEMATOPOIETIC CHIMERISM AND DONOR-SPECIFIC TOLERANCE WITHOUT IRRADIATION1

Abstract

These studies demonstrate for the first time that cytoreduction/ablation with ALS combined with sirolimus and reconstitution with donor bone marrow induces tolerance and chimerism in a completely mismatched murine combination. The use of ALS and sirolimus, currently employed therapies in clinical transplantation, and the lack of requirement for radiation make this tolerance protocol attractive for clinical application.