animal · 2010 · 12 citations · 15 references
AgricultureGeneticsMolecular GeneticsAnimal BiotechnologyGene ExpressionTransgenic Animal ModelsAnimal CloningSomatic Cell Nuclear TransferSwine GeneticsSwine ReproductionTransgenesis StrategiesTransgenic Animal TechnologyGene KnockoutNuclear Transfer TechniquesPorcine ModelsRecombinase ProteinsTransgenic Animal CloningTransgenic Animal GenerationTransgenic Livestock ProductionTransgenic Pigs
Miniature pigs have been recognized as valuable experimental animals in medical research. However, porcine models related to gene knockout of human diseases are not widely available. The objective of this study was to establish Mx1-Cre pigs using somatic cell nuclear transfer. In this study, we created transgenic pigs using somatic cell nuclear transfer (SCNT). Transfer of 210, 230, 250 and 215 zygotes to four surrogates produced 10 piglets. The Cre recombinase expression in transgenic pigs was studied using reverse transcriptase (RT)-PCR and immunohistochemistry. Mx1-Cre swine were shown to harbor the Cre gene in their genomic DNA using the PCR. In conclusion, Mx1-Cre transgenic piglets were successfully produced by SCNT. These transgenic swine, in conjunction with inducible systems for controlling Cre expression and function, are likely to have a profound impact on the study of human diseases.
15
Inducible Gene Targeting in Mice
Ralf Kühn, Frieder Schwenk, Michel Aguet et al. · Science · 1995 · 1.9K citations · Full text
Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning
Liangxue Lai, Donna Kolber‐Simonds, Kwang‐Wook Park et al. · Science · 2002 · 1.3K citations
Production of transgenic rabbits, sheep and pigs by microinjection
Robert E. Hammer, V. G. Pursel, Caird E. Rexroad et al. · Nature · 1985 · 1.1K citations
Targetted correction of a mutant HPRT gene in mouse embryonic stem cells
Thomas Doetschman, Ronald G. Gregg, Nobuyo Maeda et al. · Nature · 1987 · 792 citations