Journal of General Virology · 1987 · 35 citations · 18 references
Life SciencesImmunologyInfectious DiseasesImmunology Of Infectious DiseasesMolecular VirologyVirologyAnimal VirusesAntiviral ImmunityCell-mediated ImmunityHumoral ImmunityViral InfectionsViral VaccinesCellular Immune Response To VirusesExperimental Animal Models Of Viral InfectionImmunoaffinity PurificationT-cell Immune ResponsesT-cell Mediated ResponsesVaricella-zoster VaccinesVaricella-zoster Virus InfectionsViral Antigen Recognition
Strain 2 guinea-pigs were inoculated with infectious varicella-zoster virus (VZV) or with immunoaffinity-purified proteins of VZV. Monoclonal antibodies to the VZV gpI (90,000/58,000 complex) and to a non-glycosylated protein, p170, were used to prepare the polypeptide antigens. Humoral and cell-mediated immune responses to the infectious virus were compared with those elicited by the gpI and p170 proteins. Both VZV IgG antibody production and T lymphocyte proliferation to VZV were detected after immunization with infectious VZV and with VZV proteins. The antibody and T lymphocyte responses waned after protein immunization in comparison with the responses induced by infectious VZV but were detected again immediately after reimmunization with gpI or p170.
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