Biologically Active Protein Nanoarrays Generated Using Parallel Dip‐Pen Nanolithography

Abstract

Amine-active N-hydroxysuccinimide-terminated alkyl thiol templates are generated using parallel dip-pen nanolithography (DPN) and are used to covalently couple protein A/G. The protein arrays generated (see figure) are used to capture antibodies through affinity binding, while preserving their biological recognition properties. The versatility of the parallel DPN method for making many similar structures in a relatively high-throughput manner (14 000 dots in 10 min) is described. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2006/c0070_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.