Inhibition of Testicular Development and Feminization of the Male Genitalia by Neonatal Estrogen Treatment in a Marsupial1

Abstract

Differentiation of the mammalian gonad is thought to be constitutive, occurring independently of and not significantly altered by gonadal hormones. In this study, estradiol benzoate (EB) and testosterone propionate (TP) were administered to newborn gray opossums (Monodelphis domestica) during the first week after birth. It was found that males and females treated with TP and females treated with EB developed normally. In males treated with EB on both Day 1 and Day 3 of postnatal life, gonads did not differentiate and internal and external genitalia were feminine. These findings are discussed with respect to current theories of mammalian sexual differentiation.