Electroanalysis · 2012 · 39 citations · 23 references
EngineeringBiochemistryBiomedical EngineeringAnalytical ChemistryBiosensorsSensorsBiological SensorsClinical BiosensingElectrochemical BiosensorsElectrochemical DetectionElectrochemical SensorsNanomaterial-based BiosensorsAmperometric BiosensorsCompetitive InhibitionElectrochemical SensingGlassy Carbon ElectrodesImmunosensorsNanomaterial-based ImmunosensorsNanomaterial-modified ElectrodesNanoparticles
Abstract An amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti‐HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti‐HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6 %–15.1 % of total hemoglobin in human blood by a competitive inhibition assay.
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Standards of Medical Care for Patients With Diabetes Mellitus
Diabetes Care · 2003 · 614 citations · Full text
Advances in electrochemical immunosensors
Petr Skládal · Electroanalysis · 1997 · 191 citations