Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium <i>Deinococcus radiodurans</i> : Radiation Sensitivities of Catalase ( <i>katA</i> ) and Superoxide Dismutase ( <i>sodA</i> ) Mutants

Abstract

Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.