The Role of Zeta Potential in Rh Agglutination

Abstract

Abstract. The titration end‐points obtained with an incomplete anti‐D antiserum and Rh(D) positive test cell samples with a range of surface charge (as determined by their electrophoretic mobility), have been compared. The results suggest that although zeta potential may be a factor in the increased agglutinability of test cells, in this system it is not the one of greatest importance. It seems that reduction in surface charge by removal of N‐acetyl neuraminic acid is not sufficient to produce maximum agglutination but this can be achieved by the use of proteolytic enzyme which probably reduces steric hindrance by splitting polypeptide chains either surrounding or within the Rh antigen.