Enzymatic Elongation of the LEC14 Antigen Generates a β-1,2 Arm on N-Glycans

Abstract

Three arms carry more than two: By the total synthesis of the entire oligosaccharide, the proposed structure of the LEC14 antigen from a CHO cell line was confirmed. Surprisingly, enzymatic galactosylation of LEC14 gave the shown compound, which has a novel three-armed structural motif.