Peroxisome Proliferator-activated Receptor γ-regulated ABCG2 Expression Confers Cytoprotection to Human Dendritic Cells

Abstract

ABCG2, a member of the ATP-binding cassette transporters has been identified as a protective pump against endogenous and exogenous toxic agents. ABCG2 was shown to be expressed at high levels in stem cells and variably regulated during cell differentiation. Here we demonstrate that functional ABCG2 is expressed in human monocyte-derived dendritic cells by the activation of a nuclear hormone receptor, PPARγ. We identified and characterized a 150-base pair long functional of the human ABCG2 We the of the to that the of of ABCG2 was to in ABCG2 in dendritic cells and to a by of functional ABCG2 be exogenous endogenous activation of the PPARγ. of the ABCG2 the and of human dendritic ABCG2, a member of the ATP-binding cassette transporters has been identified as a protective pump against endogenous and exogenous toxic agents. ABCG2 was shown to be expressed at high levels in stem cells and variably regulated during cell differentiation. Here we demonstrate that functional ABCG2 is expressed in human monocyte-derived dendritic cells by the activation of a nuclear hormone receptor, PPARγ. We identified and characterized a 150-base pair long functional of the human ABCG2 We the of the to that the of of ABCG2 was to in ABCG2 in dendritic cells and to a by of functional ABCG2 be exogenous endogenous activation of the PPARγ. of the ABCG2 the and of human dendritic ATP-binding cassette cell dendritic cell dendritic as and ATP-binding cassette to a that the of a of the human ABCG2 to a of that of that to the ABCG2 is expressed in the of cell has been shown to and high of ABCG2 is in the and and was shown to a in the of the against toxic ABCG2 is expressed in stem characterized by ABCG2 is the is expressed in cell of the ABCG2 was shown to the of toxic the pump is of cell the of ABCG2 and that ABCG2 is by and the of transporters is to be regulated by nuclear hormone was that the of and the nuclear receptor, the in in cell is by the activation of of of nuclear in human dendritic cell and we identified ABCG2 as a regulated by of the Here we that and ABCG2 in a cell of the human monocyte-derived dendritic We identified and characterized the of the in We that dendritic cells by high levels of functional ABCG2 and to to the and of human dendritic cells during and and and by was by human and identified of the of the ABCG2 was to and the and cells to the and was to the was as a of was to the the was cells and of the cell We a a was was as was the as and by the in the the of that in the and by and cell as at a of and in and and the and was at to the cell the cells a of cells in of cell by in and to and was and the ABCG2 cells and at by of cells was a was was at and of was at and in that was was to and the was to of the and in of by and at to was a a to the of the was by the and of was a was a was by and and the and and of by in the and at the cells and to cells a the cells in at a of cells at was to and cells at was by the was as and as by expressed as of the and by in we characterized the of nuclear in monocyte-derived dendritic cell We that was and during and as a of and is to the the as was We to of to that of of the was the the of ABCG2 was by and monocyte-derived human the of to ABCG2 a of levels was the as the of is a to ABCG2 in be to a was to the shown in of the the of ABCG2 that is We to the of ABCG2 as shown in of and ABCG2 of ABCG2 was in a cell as by the activation of the a the in cells of the to be to a ABCG2 cells and the of in ABCG2 we to the of in We a that the of endogenous of cells of and ABCG2, that the of demonstrate that activation of ABCG2 in and in the cell to be in cell by a of has been by the of receptor, is regulated a has that and of We that a the of in that be the of the activation of ABCG2 the of we the of and the levels of and ABCG2 by shown in we that in cell the activation of to and we a of cells to and the of that in human ABCG2 is and in cells the activation of nuclear hormone in a ABCG2 is regulated by a of levels of and ABCG2 and of and the of the was by of and ABCG2 cells and at the and the was by the of the 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