Publication | Closed Access
A Simple Method of Freezing and Storing Live Lymphocytes
50
Citations
4
References
1972
Year
Storing Live LymphocytesHl—a Antibody ScreeningImmunologyHematologyLymphatic SystemImmunophenotypingHl—a TypingMedicineCell BiologyCellular PhysiologyPha Stimulation
A simple method of freezing lymphocytes requiring a cryoprotective medium consisting of glucose, MEM, and dimethylsulfoxide (DMSO) and an ultra‐low freezer to freeze and store the lymphocytes is described. The thawed lymphocytes following 2 weeks' to 5 months' storage were found to be useful for HL—A antibody screening, HL—A typing, and PHA stimulation.
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