Enzymatic Synthesis of Phosphonomethyl Oligonucleotides by Therminator Polymerase

Abstract

Therminator is the hero when it comes to the enzymatic production of phosphonomethyl–threosyl oligonucleotides with a non-natural 3′–2′ linkage (see scheme). The ability of Therminator polymerase to catalyze the condensation of diphosphate derivatives of nucleosides to give modified oligomers promises to be quite general. Such oligonucleotides are useful tools in synthetic biology because of the innate stability of the phosphonate linkage. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z603435_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.