Modular Control of Lectin Function: Redox‐Switchable Agglutination

Abstract

A specifically designed protein interface engineered into a model carbohydrate-binding lectin (GNAP71C) generates a blood-cell-binding activity that can sense ambient redox conditions (see picture; DTT=dithiothreitol, GSSG=oxidized glutathione, WT=wild-type protein, MAC=minimum agglutination concentration). The blood-cell binding exploits induced extended binding that can be varied by orders of magnitude with little change in the direct carbohydrate binding. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z53320_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.