In vitro evaluation of antimicrobial activity of combinations of nystatin and Euphorbia hirta leaf extract against Candida albicans by the checkerboard method

Abstract

The in vitro combined effects of Nystatin and methanol extract of leaves ofEuphorbia hirta against clinical isolates of Candida albicans were investigated using the Checkerboard technique. The organism was susceptible to the extract with MIC of 25 mg/ml, while Nystatin had MIC of 0.165 mg/ml against C. albicans. Upon combination, some ratios showed synergistic while others indicated indifferent activities against the isolates. These results indicate that some combinations of the extract with nystatin could be synergistic in activity for some ratio combinations and indifferent for some others.   Key words: In vitro, nystatin, Euphorbia hirta, Candida albicans, checkerboard.