Current Protocols in Molecular Biology · 2014 · 73 citations · 23 references
Life SciencesGeneticsMolecular BiologyFish BiologyGenome OrganizationGenomicsFish GenomicsFluorescence MicroscopyGenome MappingGenome VisualizationGenomic Data VisualizationOligonucleotide ChemistryBioluminescent ProbesDna ProbesFluorescent Nucleic Acid ProbesProbe-based AssaysWhole-genome Visualization
Abstract Oligopaint probes are fluorescently labeled, single‐stranded DNA oligonucleotides that can be used to visualize genomic regions ranging in size from tens of kilobases to many megabases. This unit details how Oligopaint probes can be synthesized using basic molecular biological techniques, and provides protocols for FISH, 3D‐FISH, and sample preparation. Curr. Protoc. Mol. Biol . 105:14.23.1‐14.23.20. © 2014 by John Wiley & Sons, Inc.
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