Selective Loading of Kinesin‐Powered Molecular Shuttles with Protein Cargo and its Application to Biosensing

Abstract

A new shuttle mission: The design of molecular shuttles, which integrate the motor protein kinesin to propel microtubules (the associated polymeric filament), requires a general strategy to achieve selective cargo binding. We describe the in situ functionalization of biotinylated microtubules with biotinylated antibodies via a streptavidin bridge and demonstrate that selective binding of antigens can be detected via a double-antibody sandwich assay on the moving shuttle.