PubMed · 1996 · 107 citations · 33 references
Life SciencesCell BiologyMolecular BiologyMolecular Cell BiologyMolecular OncologyOncogenesCancer Cell SignalingCell Cycle Gene RegulationCell Cycle SignalingCell-cycle RegulationCyclin-dependent KinasesTumor Suppressor GenesCell Cycle DynamicsCyclinsG1 Phase ControlG1 Phase ProgressionG1 TransitionG1-s Phase TransitionG1/s TransitionRetinoblastoma Family Proteins
The retinoblastoma (Rb) family consists of the tumor suppressor pRb and related proteins p107 and pRb2/p130. Ectopic expression of pRb and p107 results in a growth arrest of sensitive cells in the G1 phase of the cell cycle. We demonstrated here that the growth-suppressive properties of pRb2/p130 were also specific for the G1 phase. The A-, E-, and D-type cyclins as well as transcription factor E2F1 and the E1A viral oncoprotein were able to rescue the pRb2/p130-mediated G1 growth arrest in SAOS-2 cells. The rescue with cyclins A and E correlated with their physical interaction with pRb2/p130, which surprisingly has been found to occur over all phases of the cell cycle. The phosphorylation status as well as the kinase activity associated with pRb2/p130 dramatically increased near the G1-S-phase transition. This suggests that, like the other Rb family members, pRb and p107, the phosphorylation of pRb2/p130 is controlled by the cell cycle machinery and that pRb2/p130 may indeed be another key G1-S-phase regulator.
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The E2F transcription factor is a cellular target for the RB protein
Srikumar Chellappan, Scott W. Hiebert, Maria Mudryj et al. · Cell · 1991 · 1.4K citations