Real-Time Fluorescence-Based PCR for Detection of Malaria Parasites

May-Ann Lee, Chiew-Hoon Tan, Lay-Tin Aw, Choon-Siang Tang, Mulkit Singh, Sze-Hee Lee, Hwee-Pin Chia, P. H. Yap

Journal of Clinical Microbiology · 2002 · 88 citations · 10 references

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Concepts

Abstract

A fluorescence-based real-time 5' nuclease PCR capable of detecting all four human malaria parasites was developed to screen large numbers of samples during an outbreak to prevent further transmission of malaria. The effectiveness of antimalarial therapy for malaria patients can be monitored by determining the reduction of parasitemia by this method.

References

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