Localization and differential regulation of angiotensinogen mRNA expression in the vessel wall.

Abstract

Recent data demonstrate the existence of a vascular renin an- giotensin system. In this study we examine the localization of angiotensinogen mRNA in the blood vessel wall of two rat strains, the Wistar and Wistar Kyoto (WKY), as well as the regulation of vascular angiotensinogen mRNA expression by dietary sodium. Northern blot analysis and in situ hybridization histochemistry demonstrate that in both strains angiotensino- gen mRNA is detected in the aortic medial smooth muscle layer as well as the periaortic fat. In WKY rats fed a 1.6% sodium diet, angiotensinogen mRNA concentration is 2.6-fold higher in the periaortic fat than in the smooth muscle, as analyzed by quantitative slot blot hybridization. Angiotensinogen mRNA expression in the medial smooth muscle layer is sodium regu- lated. After 5 d of a low (0.02%) sodium diet, smooth muscle angiotensinogen mRNA levels increase 3.2-fold (P < 0.005) as compared with the 1.6% sodium diet. In contrast, angiotensinogen mRNA level in the periaortic fat is not influenced by so- dium diet. In summary, our data demonstrate regional (smooth muscle vs. periaortic fat) differential regulation of angiotensin- ogen mRNA levels in the blood vessel wall by sodium. This regional differential regulation by sodium may have important physiological implications. ( J. Clin. Invest.