A Fruiting Body Tissue Method for Efficient <i>Agrobacterium</i> -Mediated Transformation of <i>Agaricus bisporus</i>

Abstract

We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.