Overexpression, Purification, Characterization, and Pathogenicity of <i>Vibrio harveyi</i> Hemolysin VHH

Yingbin Zhong, Xiao‐Hua Zhang, Jixiang Chen, Zhenghao Chi, Bo-guang Sun, Yun Li, Brian Austin

Infection and Immunity · 2006 · 62 citations · 30 references

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Abstract

Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

References

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