Liquid Chromatography of Enantiomers: Determination of Enantiomeric Purity in Spite of Extensive Peak Overlap

Abstract

A new approach to the determination of enantiomeric purity P is based on the known technique of liquid chromatography on chiral phases, such as triacetyl‐cellulose. Interfering overlapping or fusion of peaks are overcome by simultaneous measurement of absorbance A and rotation α as functions of the volume eluted (υ)· C + is determined graphically. equation image