Collisionally activated dissociation of peptides using a quadrupole ion‐trap mass spectrometer

Abstract

Abstract The ion‐trap mass spectrometer can be used to record mass and multi‐stage tandem mass spectra (MS n ) on small peptides. The compounds are ionized by Cs + surface ionization, injected into the trap, mass selected, activated by collision, resulting in dissociation and mass‐selectively ejected. Tandem mass spectrometric data on sub‐fmol amounts of Gramicidin S are shown, along with tandem mass spectrometric and MS n data on two other peptides. The experiment is distinguished by its very high sensitivity and the high quality of sequence information obtained.