Antimicrobial Agents and Chemotherapy · 2002 · 325 citations · 19 references
Life SciencesGeneticsMicrobiologyAntimicrobial ResistanceGenomicsMicrobial GeneticsAntibiotic Resistance GeneticsBeta-lactam ResistanceHorizontal Gene TransferPlasmid BiologyPlasmid GeneticsResistance GenesAntibiotic Resistance PlasmidsBeta-lactamase EvolutionBeta-lactamase Gene TransferBeta-lactamase GenesCarbapenem Resistance PlasmidsMobile Genetic Element AnalysisPlasmid ClassificationPlasmid-mediated Beta-lactam Resistance
Kluyvera ascorbata produces a beta-lactamase that results in an atypical susceptibility pattern, including low-level resistance to penicillins, cephalothin, and cefuroxime, but this resistance is reversed by clavulanate. Ten nucleotide sequences of the corresponding gene, bla(KLUA), were obtained and were found to have minor variations (96 to 100%). Otherwise, bla(KLUA) was found to be similar (95 to 100%) to some plasmid-encoded CTX-M-type beta-lactamases. Finally, mobilization of bla(KLUA) on a plasmid was found to be mediated probably by a genetic mobile element like ISEcp1.
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