Publication | Closed Access
Determination of the glycoalkaloid content of potato tubers by three methods including enzyme‐linked immunosorbent assay
27
Citations
12
References
1985
Year
BotanyGeneticsGlycobiologyPlant MetabolomicsApplied GeneticsFood ChemistryAgricultural ChemistryBioanalysisGlycoalkaloid ContentTotal Glycoalkaloid ContentPhytochemicalChromatographyAllergyAgricultural BiotechnologyPotato TubersPharmacologyPlant BreedingConventional Chemical AssaysEnzyme‐linked Immunosorbent AssayPhytochemistryMedicine
Abstract Potato tuber samples of diverse genetic backgrounds were analysed independently for total glycoalkaloid content by three different procedures. Two of the methods used were conventional chemical assays, the third an enzyme‐linked immunosorbent assay (ELISA). Agreement between all three was extremely good, though the ELISA employed much simpler sample preparation, was technically easier to carry out, and could assay more rapidly large numbers of samples. For these and other reasons, the ELISA will find wider application in future.
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