Systemic Induction of Peroxidases, 1,3-β-Glucanases, Chitinases, and Resistance in Bean Plants by Binucleate<i>Rhizoctonia</i>Species

Abstract

ABSTRACT Inoculation of bean hypocotyls with a nonpathogenic binucleate Rhizoctonia (BNR) species induced systemic resistance and protection of the roots and cotyledons to later challenge with the root rot pathogen Rhizoctonia solani or the anthracnose pathogen Colletotrichum lindemuthianum. Bean seedlings that were treated with BNR 48 h prior to their challenge with R. solani or C. lindemuthianum had few necrotic lesions and reduced disease severity as compared with seedlings not treated with BNR. Treatment with BNR 48 h prior to their challenge also elicited a significant and systemic increase in all cellular fractions of peroxidases, 1,3-beta-glucanases, and chitinases compared with the diseased and control plants. Compared with control plants, total peroxidases and glucanases increased twofold and eightfold, respectively, in all protected bean tissues. BNR 232-CG could not be recovered from the challenged hypocotyls or cotyledons, indicating that there was no contact between the inducer and the pathogen. Both the 1,3-beta-glucanases and the peroxidases were positively correlated with induced resistance.