Applied and Environmental Microbiology · 2006 · 40 citations · 28 references
Life SciencesBiochemistryMicrobiologyMetabolismMicrobial MetabolismMicrobial PhysiologyBacterial CultivationMicrobial BioenergeticsMicrobial GrowthNitrogen Limitation ResponsePolyhydroxyalkanoate ProductionPolyhydroxyalkanoatesPolyphosphate-accumulating OrganismsPseudomonas SpeciesRate Limiting
Pseudomonas putida CA-3 accumulates polyphosphate (polyP) and medium-chain-length polyhydroxyalkanoate (mclPHA) concurrently under nitrogen limitation. Five other mclPHA-accumulating Pseudomonas strains are capable of simultaneous polyP and mclPHA biosynthesis. It appears that polyP is not the rate-limiting step for mclPHA accumulation in these Pseudomonas strains.
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Measurement of protein using bicinchoninic acid
Pam Smith, Randall I. Krohn, Greg T. Hermanson et al. · Analytical Biochemistry · 1985 · 18.4K citations
Arnulf Timm, Alexander Steinbüchel · Applied and Environmental Microbiology · 1990 · 339 citations · Full text