3′‐(2,4‐Dinitrobenzenesulfonyl)‐2′,7′‐dimethylfluorescein as a Fluorescent Probe for Selenols

Abstract

Simply the BESThio: The fluorescein derivative BESThio (see scheme) operates as a thiol probe at pH 7.4 and functions as a selenol probe at pH 5.8, and can thus be selectively applied to the rapid quantification of selenocysteine and the determination of the selenocysteine content in selenoproteins such as glutathione peroxidase and thioredoxin reductase with high sensitivity. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z504299_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.