Investigation of sucrose synthase from rice for the synthesis of various nucleotide sugars and saccharides

Abstract

The unique character of the plant glucosyltransferase sucrose synthase, to catalyse in vitro the synthesis and cleavage of sucrose under appropriate conditions, can be exploited for the enzymatic synthesis of carbohydrates. The present paper describes the potential utilization of sucrose synthase from rice for the enzymatic synthesis of activated sugars and saccharides. In the cleavage reaction of sucrose, the nucleoside diphosphates can be used in the order UDP > TDP > ADP > CDP > GDP to obtain the corresponding activated glucoses. In batch reactions, > 90% conversion of UDP and TDP could be achieved. Substituting different di- and trisaccharides for sucrose in the cleavage reaction with UDP 2-deoxysucrose was the most promising substrate. Sucrose synthase was combined with UDP-galactose 4'-epimerase and beta 1-4 galactosyltransferase to synthesize N-acetyllactosamine with in situ regeneration of UDP-glucose. In the synthesis reaction of sucrose synthase, different donor (UDP-sugars) and acceptor substrates were investigated. UDP-N-acetylglucosamine and UDP-xylose could be used in combination with fructose as acceptor. D-Xylulose, D-tagatose, D-lyxose, D-psicose, L-sorbose, D-mannose, L-arabinose, 1,6 anhydroglucose, lactulose, raffinose and isomaltulose can serve as acceptors for UDP-glucose.