An enzyme‐linked immunosorbent assay for diacetoxyscirpenol applied to the analysis of wheat

Abstract

Abstract High‐titre, high‐specificity antisera against diacetoxyscirpenol have been produced in rabbits and used to set up an indirect, microtitration‐plate enzyme‐linked immunosorbent assay for the trichothecene mycotoxin. The assay, with a limit of detection of 2 pg per well, has been applied to the determination of diacetoxyscirpenol in wheat. Cereal extraction consists of a simple solubilisation of toxin in methanol resulting in an assay sensitivity of 300 ng g −1 . Recovery, measured at three different toxin levels, was essentially quantitative.