Methanol dehydrogenase from Hyphomicrobium MS 223

Abstract

The monomethyl sulfate-degrading bacterium, Hyphomicrobium MS 223, contains a NAD(P)-independent methanol dehydrogenase (EC 1.1.99.8) which was isolated and characterized. The enzyme was activated by ammonium ions, had an Mr of 118000 and was composed of two subunits of identical Mr. It showed a broad substrate specificity for primary alcohols and was able to oxidize secondary alcohols and several aliphatic aldehydes. The new competitive inhibitor acetaldehyde oxime inhibited aldehyde oxidation more strongly than alcohol oxidation.