A simple technique for large scale in vitro culture of Plasmodium falciparum.

Abstract

The large scale in vitro cultivation method of Fairlamb et al (1985) was modified to contain human plasma-supplemented medium in HEPES buffer. After 4 days with no change of medium nor agitation of the culture flask, 27- to 50-fold increase in the starting parasitemia of trophozoites were obtained.