Chemical Communications · 2011 · 75 citations · 13 references
Life SciencesBiotechnologyMolecular BiologyGene ExpressionMolecular BiotechnologySynthetic BiologyMacromolecular AssemblyProtein Expression EngineeringProtein SynthesisSynthetic Gene CircuitsTransgene Expression ControlTranslational RegulationRiboswitch DesignRiboswitch EngineeringRiboswitch RegulationRiboswitch-mediated RegulationRna Regulatory CircuitsSynthetic RiboswitchesWater-in-oil Emulsions
Synthetic riboswitches can be used to control protein expression under fully defined conditions in vitro, in water-in-oil emulsions, and in vesicles. The developed system could serve as a foundation for the construction of cellular mimics that respond to molecules of our choosing.
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Cell-free translation reconstituted with purified components
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