Plant Tissue Culture and Biotechnology · 1970 · 28 citations · 12 references
AgriculturePlant BiologyPlant SciencesOrganogenesisPlant NutritionPlant PropagationMedicinal Plant ProductionPlant EstablishmentPropagation MediaPropagation Of Rare PlantsTissue CultureVegetative PropagationCallus CultureCallus-based InductionCallus-induced RegenerationIndirect OrganogenesisNodal Shoot DevelopmentShoot Culture
An efficient protocol was developed for in vitro propagation of Abrus precatorius L. through induction of indirect organogenesis in nodal segment derived callus tissue. Yellowish-green nodular callus was induced at the cut surface of the nodal segments cultured on MS fortified with 5.0 mg/l BAP and 0.5 mg/l NAA. The callus differentiated into adventitious shoots when it was subcultured on to MS supplemented with 3.0 mg/l BAP + 0.5 mg/l Kn + 0.5 mg/l NAA. On an average 6.87 ± 0.26 shoots/culture developed. These microshoots were rooted in half-strength MS containing 1.0 mg/l IBA and the rooted plantlets were transferred to soil after proper acclimatization.Key words: Abrus precatorius, Medicinal plant, Callus culture, PropagationDOI = 10.3329/ptcb.v17i1.1121Plant Tissue Cult. & Biotech. 17(1): 59-64, 2007 (June)
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