Butyramide-utilizing Mutants of Pseudomonas aeruginosa 8602 which Produce an Amidase with Altered Substrate Specificity

Abstract

SUMMARY Mutants of Pseudomonas aeruginosa 8602 were isolated which, unlike the wild type, were able to grow with butyramide as a carbon source. Six mutants derived from the constitutive strain c 11 were shown to produce an enzyme (B amidase) with altered electrophoretic mobility and altered substrate specificity. The apparent K m for butyramide of the B amidase was about a tenth of that of the A amidase and the V max was about ten-fold greater. A further mutation produced mutants able to grow on valeramide.