Protein and Peptide Letters · 2002 · 14 citations · 0 references
Life SciencesBiochemistryNeuroscienceMembrane BiophysicsMolecular NeurobiologyNeurodegenerationAlzheimer DiseaseAmyloid FormationBiological MembranesMembrane FluidityMembrane PermeabilityPermeability AssessmentAmyloid Beta AggregationAmyloid Beta PeptidesAmyloid Fibril FormationAmyloid FibrillizationAmyloid FibrilsFluorescence ProbesFluorescent ProbesNeurotoxicity
The time course of A beta fibril formation was characterized using a variety of assays. The effect of A beta on the membrane fluidity and permeability was assessed by monitoring the anisotropy of the fluorescent probe DPH and TMA-DPH and measuring the release of vesicle-entrapped with three different molecular weight fluorescence probes (ANTS/DPX, Calcein, FD-4). The results show fibrils had formed after 4 days. Aggregated A beta may decrease the membrane fluidity and induce the leakage of ANTS and Calcein in a dose dependent manner. These effects may have some relation with A beta neurotoxicity.