Optimization of glucose oxidase production by Aspergillus niger

Abstract

-1 ml -1 ) was achieved at 10% glucose concentration after 48 h of submerged fermentation. The pH for the optimal production of enzyme was found to be 5.5 (enzyme activity 1.56 µmoleHQMin -1 ml -1 ). Addition of urea (0.2%) and KH 2PO 4 (0.4%) into the fermentation medium increases enzyme activity (2.01 and 2.96 µmoleHQMin -1 ml -1 , respectively) while MgSO 4·7H 2O was found to inhibit