Selective Detection of 5‐Methylcytosine Sites in DNA

Abstract

A distinguished result: 5-Methylcytosine can be distinguished from all other nucleobases in DNA by selective conversion of the C5C6 double bond into a bromohydrine followed by piperidine-induced strand cleavage at the reaction site (see picture). This procedure allows the methylation status of the p16 promotor to be determined under in vitro conditions.