Crinamine from Crinum asiaticum var. japonicum Inhibits Hypoxia Inducible Factor-1 Activity But Not Activity of Hypoxia Inducible Factor-2

Abstract

In a search for natural product inhibitors of hypoxia inducible factor-1 (HIF-1) function, crinamine (1), a crinane type alkaloid, showed potent dose dependent inhibition (IC50 = 2.7 microM) of HIF-1alpha in a cell-based reporter gene assay. Crinamine (1) was isolated from the aerial parts of Crinum asiaticum var. japonicum together with lycorine (2), norgalanthamine (3) and epinorgalanthamine (4). The other components (2-4) showed no significant inhibition of HIF-1alpha induced transcriptional activity.