A Method for Trapping Intermediates of Polyketide Biosynthesis with a Nonhydrolyzable Malonyl‐Coenzyme A Analogue

Abstract

Trapped in the middle: To investigate the mechanistic details of polyketide biosynthesis, intermediates are trapped by a nonhydrolyzable malonyl-coenzyme A analogue that reacts with a growing polyketide chain formed by a stilbene synthase. As the reverse transesterification reaction is not possible, the polyketide intermediates accumulate attached to the analogue (see scheme; Enz=enzyme, CoA=coenzyme A).