Cloning and characterization of the 5-aminolevulinate synthase gene(s) from Rhodobacter sphaeroides

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

Life Sciences, Genetics, Molecular Genetics +12

A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples

Mary Ann K. Markwell, Suzanne M. Haas, L.L. Bieber et al. · Analytical Biochemistry · 1978 · 6.5K citations

Life Sciences, Biochemistry, Protein Biochemistry +13

Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of <i>Escherichia coli</i> by R-Factor DNA

Stanley N. Cohen, Annie Chang, Leslie Hsu · Proceedings of the National Academy of Sciences · 1972 · 2.7K citations

Life Sciences, Genetics, Microbiology +15