High performance liquid chromatography of triglycerides: Controlling selectivity with reverse phase columns

Abstract

Abstract Rapid separations of triglycerides by chain length and degree of unsaturation have been made by high performance liquid chromatography (HPLC) using reverse phase columns. Several different bonded columns were evaluated for use in reverse phase HPLC of triglycerides, and differences in selectivity are discussed. Selectivity was also modified by adding silver ion to the solvent, which produces marked changes in selectivity of solute triglycerides.