BMB Reports · 2008 · 20 citations · 27 references
Life SciencesGeneticsMolecular BiologyCell CycleCell DivisionGene ExpressionCell-cycle ProgressionMitosisPlant Cell Cycle RegulationPlant Gene ExpressionReplication In EukaryotesReplication InitiationCell Division ControlDifferential Transcript ExpressionPre-replication Complex AssemblyProtein Expression LevelsPull-down AssaysReplication Complex AssemblyTranscript-level Expression AnalysisYeast Cell Cycle Genetics
The pre-replication complex (pre-RC), including the core hexameric MCM2-7 complex, ensures that the eukaryotic genome is replicated only once per cell division cycle. In this study, we identified a rice minichromosome maintenance (MCM) homologue (OsMCM2) that functionally complemented fission yeast MCM2 (CDC19) mutants. We found OsMCM2 transcript expression in roots, leaves, and seeds, although expression levels differed slightly among the organs. Likewise, the OsMCM2 protein was ubiquitously expressed, but it was downregulated when nutritients were limiting, indicating that MCM2 expression (and therefore cell cycle progression) requires adequate nutrition. Yeast two-hybrid and GST pull-down assays demonstrated that OsMCM2 interacted with the COP9 signalosome 5 (CSN5). Taken as a whole, our results indicated that OsMCM2 functions as a subunit of the rice MCM complex and interacts with CSN5 during developmental regulation.
27
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
R. Daniel Gietz, Robert H. Schiestl, Andrew Willems et al. · Yeast · 1995 · 2.1K citations