Proceedings of the National Academy of Sciences · 1983 · 57 citations · 25 references
Life SciencesGeneticsMolecular GeneticsBiochemical GeneticsHuman GeneticsMedical GeneticsGenetic VariationGenotype-phenotype Association AnalysisGenotype-phenotype CorrelationProtein ElectrophoresisVariant ClassificationVariant DetectionGenetic PolymorphismsGenetic Variant DetectionHeterozygosity EstimationPolyacrylamide Gel ElectrophoresisTwo-dimensional ElectrophoresisTwo-dimensional Gel Electrophoresis
A series of 62 plasma samples have been examined for genetic variation by the technique of two-dimensional polyacrylamide gel electrophoresis followed by silver-staining of the gels. Twenty polypeptides chosen without respect to variability were considered suitable for scoring. Of the total of 1,240 polypeptides, 29 could not be scored unambiguously. Seventy-five of the remaining 1,211 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subject. This index of heterozygosity (6.2 +/- 0.7%) is substantially higher than those reported by others in similar studies of human fibroblasts, lymphocytes, kidney, or brain cells.
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Silver staining of proteins in polyacrylamide gels
Wayne Wray, Teni Boulikas, Virginia P. Wray et al. · Analytical Biochemistry · 1981 · 3.5K citations