Transactions of the American Fisheries Society · 1982 · 30 citations · 0 references
Natural ResourcesMicrobiologyToxicologyAquacultureFish BiologyFish PathologyBacterial ToxinsFish Disease EtiologyFish HealthFish ImmunopathologyFish ToxicologyMarine BiotoxinsBacterial ExotoxinsEndotoxin ToxicityFish Disease PathogenesisIntraperitoneal AdministrationLethal Concentration AssessmentLethal Concentration MedianMedian Lethal DoseShellfish Biotoxins
The effects of the extracellular products (ECP) and endotoxin from Aeromonas hydrophila grown in a defined medium were determined following intraperitoneal injection of young-of-the-year channel catfish Ictalurus punctatus. Endotoxin had no lethal effects in doses as high as 400 μg per 7.2-g fish, whereas ECP had a 48-hour median lethal concentration (LD50) of 15.7 μg per 7.2-g fish. Sublethal doses of ECP often produced many of the gross pathologies associated with A. hydrophila infections. The primary lethal factor was heat-labile at 56 C for 10 minutes, but a heat-stable component was also implicated.