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Scattering spectroscopy of molecules at nanometer resolution
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1996
Year
EngineeringMicroscopyNanometer ResolutionMolecular BiologyLight Scattering SpectroscopyMolecular Spatial ResolutionMicroscopy MethodLight MicroscopyMolecular ImagingMolecular SpectroscopyBiophysicsNew FormImagingBiophotonicsSingle-molecule DetectionFluorescence MicroscopySpectroscopyBiomedical ImagingLight ScatteringLocal ScatteringMedicine
A new form of optical spectroscopy is demonstrated whereby the local scattering from the interaction between a nanometer size probe tip and a sample is measured as a function of wavelength. We show images of viruses and molecules at molecular spatial resolution and demonstrate how the image contrast varies with wavelength. To first approximation, the contrast as a function of wavelength varies in the same way as the local polarizability of the sample