A Single-Stage Polymerase-Based Protocol for the Introduction of Deletions and Insertions Without Subcloning

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction

Kary B. Mullis, Fred Faloona, S. Scharf et al. · Cold Spring Harbor Symposia on Quantitative Biology · 1986 · 3.5K citations

Genetics, Dna Analysis, Molecular Biology +20

The "megaprimer" method of site-directed mutagenesis.

Gobinda Sarkar, S S Sommer · PubMed · 1990 · 1.2K citations

Engineering, Genetics, Oligonucleotide Primers +18

Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange ^TM Site-Directed Mutagenesis

Wenyan Wang, Bruce A. Malcolm · BioTechniques · 1999 · 598 citations · Full text

Integration of PCR Fragments at Any Specific Site within Cloning Vectors without the Use of Restriction Enzymes and DNA Ligase

Martin Geiser, Régis Cèbe, Delia Drewello et al. · BioTechniques · 2001 · 249 citations · Full text

Engineering, Genetics, Dna Analysis +21

Generation of Deletion and Point Mutations with One Primer in a Single Cloning Step

Olga V. Makarova, Emmanuel Kamberov, Ben Margolis · BioTechniques · 2000 · 222 citations · Full text

Point Mutations, Genetics, Genetic Engineering +9