Identification of a Glycosyltransferase from Mycobacterium marinum Involved in Addition of a Caryophyllose Moiety in Lipooligosaccharides

Abstract

Deletion of Mycobacterium marinum MMAR2333 resulted in the loss of three of four subclasses of lipooligosaccharides (LOSs). The mutant was unable to extend an intermediate (LOS-II*) by addition of caryophyllose. These data and the predicted domain structure suggest that MMAR2333 is a glycosyltransferase involved in the generation of a lipid-linked caryophyllose donor.