physica status solidi (a) · 2003 · 25 citations · 0 references
PharmacyBiochemistryMolecular BiologyBiophysical ChemistryNucleic Acid BiochemistryNucleic Acid ChemistryDna ChemistryDna StabilityNucleic Acid BiophysicsNucleic Acid LabelingNucleic Acid ModificationConfocal Fluorescence MicroscopyConfocal Microscopy VisualizationCovalent ImmobilizationDenaturationDna FunctionalizationDna ImmobilizationImmobilizationNon-specific Dna Binding
Chemical vapour deposited (CVD) diamond is used in DNA immobilization experiments for its high stability and versatility. In this study a double-stranded, 250 base pairs long DNA fragment of the human PKU gene was covalently bound to CVD diamond films. Thymidine was used as a linker molecule for the binding in head-on configuration. The presence of surface-bound FITC-labelled DNA was confirmed by confocal fluorescence microscopy. In denaturation experiments the second, unlabelled strand of each pair was removed resulting in a loss of fluorescence. The possibility of non-specific binding of DNA to diamond can be excluded. (© 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)