An improved suicide vector for construction of chromosomal insertion mutations in bacteria

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria

Reinhard Simon, U. B. Priefer, Alfred Pühler · Bio/Technology · 1983 · 7.3K citations

Life Sciences, Genetics, Molecular Genetics +12

A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR

Virginia L. Miller, John J. Mekalanos · Journal of Bacteriology · 1988 · 2.1K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria

Marta Herrero, Vı́ctor de Lorenzo, Kenneth N. Timmis · Journal of Bacteriology · 1990 · 1.6K citations · Full text

Life Sciences, Biotechnology, Genetics +17

A wide-host-range suicide vector for improving reverse genetics in Gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica

Koné Kaniga, Isabelle Delor, Guy R. Cornelis · Gene · 1991 · 717 citations

Life Sciences, Genetics, Microbiology +7